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28:13
YouTube
the bumbling biochemist
Overview of methods to measure biochemical binding affinity
Some of the methods covered include: • Surface Plasmon Resonance (SPR) • Bio-Layer Interferometry (BLI) • Isothermal Titration Calorimetry (ITC) • Microscale Thermophoresis (MST) • Fluorescence Polarization (FP) •Slot blots & dot blots •EMSA (Electrophoretic Mobility Shift Assay) •ELISA (Enzyme-Linked Immunosorbent Assay ...
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